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KMID : 0816120120150020085
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Korean Journal of Pediatric Gastroenterolology and Nutrition
2012 Volume.15 No. 2 p.85 ~ p.90
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Comparison of Four Commercial ELISA Kits and In-House Immunoblotting for Diagnosis of Helicobacter pylori Infection
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Jeong Hoar-Lim
Jung Yang-Sook
Jun Jin-Su
Yeom Jung-Sook
Park Ji-Sook
Seo Ji-Hyun
Lim Jae-Young
Park Chan-Hoo
Woo Hyang-Ok
Youn Hee-Shang
Ko Gyung-Hyuck
Baik Seung-Chul
Lee Woo-Kon
Cho Myung-Je
Rhee Kwang-Ho
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Abstract
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Purpose: Commercial enzyme-linked immunosorbent assay (ELISA) kits have been considered less reliable for children than for adults. The aim of this study was to compare four ELISA kits and in-house immunoblotting based on the analysis of anti-H. pylori -IgG antibody reactivity.
Methods: A total of 399 serum samples were collected at the GNU Hospital during 1998?1999. All sera were tested using ELISA and immunoblotting. Statistically significant differences were determined by the χ2 test.
Results: The overall seropositivity rates using GAP IgG, Genedia IgG, HM-CAP, Pyloriset EIA-G, and immunoblotting were 13.0%, 25.1%, 18.3%, 15.8%, and 62.9%, respectively. Immunoblotting showed a higher seropositivity rate than did all four ELISA kits in all age groups. Genedia IgG had the highest seropositivity among the ELISA kits. The seropositivity rate for children aged 13 to 18 months was lowest, and that of children aged 15 years was highest (90.0%). The seropositivity rate for children aged 7 months to 5 years was significantly lower than that for children aged 6 to 15 years among the four ELISA kits (p＜0.0001) and immunoblotting (p=0.02).
Conclusion: Immunoblotting is the most sensitive test for detection of anti-Helicobacter pylori IgG antibodies among the serological tests in this study. These results emphasize the need for standardization when commercial ELISA tests are used in different nations or in young age groups. Immunoblotting could be a suitable noninvasive assay for serodiagnosis and seroepidemiologic study of H. pylori infection in Korean children.
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KEYWORD
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ELISA, Immunoblot, Helicobacter pylori infection, Children
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